Ladomery Lab RNA Biology Group

Ladomery Lab
Southwest UK RNA Club

Research Papers

WT1 interacts with the splicing protein RBM4 and regulates its ability to modulate alternative splicing in vivo

Exp. Cell Res. 312: 3379-3388 (2006) - PMID: 16934801

Authors: Markus MA, Heinrich B, Raitskin O, Adams DJ, Mangs H, Goy C, Ladomery M, Sperling R, Stamm S, Morris BJ.


Wilms tumour protein 1 (WT1), a protein implicated in various cancers and developmental disorders, consists of two major isoforms: KTS">WT1, a transcription factor, and WT1, a posttranscriptional regulator that binds to RNA and can interact with splicing components. Here we show that WT1 interacts with the novel splicing regulator RBM4. Each protein was found to co-localize in nuclear speckles and to co-sediment with supraspliceosomes in glycerol gradients. RBM4 conferred dose-dependent and cell-specific regulation of alternative splicing of pre-mRNAs transcribed from several minigenes. We found that overexpressed WT1 abrogated this effect of RBM4 on splice-site selection, whereas KTS">WT1 did not. We conclude that the (+KTS) form of WT1 is able to inhibit the effect of RBM4 on alternative splicing.

Keywords: Alternative splicing; RNA binding proteins; speckles; Wilms tumour suppressor; supraspliceosome