Ladomery Lab RNA Biology Group


Ladomery Lab
Southwest UK RNA Club

Research Papers

The Wilms' tumor 1 (WT1) gene (+KTS isoform) functions with a CTE to enhance translation from an unspliced RNA with a retained intron

Genes & Dev. 20: 1597-1608 (2006) - PMID: 16738405

Authors: Bor YC, Swartz J, Morrison A, Rekosh D, Ladomery M, Hammarskjöld ML

Abstract

The Wilms’ tumour 1 (WT1) gene plays an important role in mammalian urogenital development, and dysregulation of this gene is observed in many human cancers. Alternative splicng of WT1 RNA leads to the expression of two major protein isoforms, WT1 and KTS">WT1. Whereas KTS">WT1 acts as a transcriptional regulator, no clear function has been ascribed to WT1, despite the fact that this protein is crucial for normal development. Here we show that WT1 (+KTS) functions to enhance expression from RNA possessing a retained intron and containing either a cellular ot viral constitutive transport element (CTE). WT1 expression increases the levels of unspliced RNA containing a CTE and specifically promotes the association of this RNA with polyribosomes. These studies provide further support for links between different steps in RNA metabolism and for the existence of post-transcriptional operons.

Keywords: WT1,Wilms tumour; CTE,constitutive transport element; RNA export; post-transcriptional regulation; translational regulation